The stages of cellular respiration include glycolysis, pyruvate oxidation, the citric acid or Krebs cycle, and oxidative phosphorylation. Introduction Cellular respiration is one of the most elegant, majestic, and fascinating metabolic pathways on earth.
Enzyme mediated reactions are required. The equation for cellular respiration is: The consumption of oxygen, which will tell you how many moles of oxygen are consumed during cellular respiration.
That is what was measured in this Lab 6 cellular respiration. The production of CO2 can also be measured. And of course the release of energy can be measured. Cellular respiration is a catabolic pathway and the mitochondria houses most of the metabolic equipment for cellular respiration.
It will break down glucose in what we call an exergonic reaction. Like previously said, the consumption of oxygen molecules will be measured in a gas form. One must know the physical laws of gases when working with them.
The laws are summarized by the following equation. If the water temp and volume stay constant then the water will move toward the region of lower pressure. During respiration, oxygen will be consumed and its volume will be reduced because the CO2 is being converted to a solid.
The net result is a decrease in gas volume in the tube and a decrease in pressure of the tube. The vial with beads will detect any atmospheric changes. Hypothesis Several different things will affect the rate of O2 consumption. The non germinating peas will have a lower rate than the germinating peas and the coldness of the water will slow the rates.
Materials The materials used for this lab were: Obtain a mL graduated cylinder and fill it with 50 mL of H2O. Drop in 25 germinating peas. Determine the amount of water displaced.
Take peas out and place on paper towel. Drop 25 dry peas into the cylinder. Add glass beads to obtain the same volume that you got in respirometer 1. Remove peas and beads to a paper towel.
Add 50 mL of water to the cylinder. Put only beads in to get an equivalent volume to the first 2 respirometers. Put on paper towel when finished. Repeat respirometer 1 steps for respirometer 4.
And 2 for 5. And 3 for 6. Listen to your teacher on how and where to set up the respirometers. Now fill your vials with the required items shown in the table and in figure 5.
Seal the vials after your items have been put in to stop any gas or water leaks. Place a weighted collar onto the bottom of your vials so they will stay submerged in the water baths. During equilibration use masking tape attached to each side of the water baths to hold the respirometers out of water for 7 minutes.
Finally submerge totally the respirometers and let them equilibrate for 3 more minutes. Read the water line where the oxygen is and record in intervals of 5 minutes all the way up to 25 minutes.
Record in table 5.Students will use a respiration chamber to measure and record the rate of oxygen consumption (cellular respiration) using germinating seeds versus a non-germinating control sample.
Teachers manual and Student Study Guide copymasters are included. There are enough materials provided for 8 lab groups. Meets AP Science. Lab Resources; Course Content by Unit AP Biology Laboratory Resources.
On this page you will find helpful links and resources to help you complete the 13 AP Biology investigative labs. Lab 6: Cellular Respiration.
The purpose of this lab is to investigate glycolysis, the Krebs cycle and electron transport. Additional information is given in Chapter 7 of your text. II. THE FOUR STAGES OF OXIDATIVE RESPIRATION OF GLUCOSE. The first, glycolysis, splits glucose into two 3-carbon molecules called pyruvate. Two ATP and two NADH are formed in the process. Which reactant is the source of energy for the process of cellular respiration? 5. What are the products (substances made) during cellular respiration? 6. Model the products (substances made) in cellular respiration by rearranging the beads in the box on the left side of the Photosynthesis and Cellular Respiration sheet. 7. Introduction Cellular respiration is a process that most living organisms undergo to create and obtain chemical energy in the form of adenosine triphosphate (ATP). The energy is synthesized in three separate stages of cellular respiration: glycolysis, citric acid cycle, and the electron transport chain.
Use a respirometer to measure the rate of cellular respiration in a living organism. Download the pdf file of the lab here. Lab 6. Cellular Respiration: How Does the Type of Food Source Affect the Rate of Cellular Respiration in Yeast?
Introduction One characteristic of living things is they must take in . Randy Kwon Mr. Peralla Biology Block 3 14 November Cellular respiration Purpose Statement The purpose of this lab is to see if there is a relationship between the amount of Carbon dioxide produced and the amount of exertion/exercise in relation to Cellular respiration.
Lab 5 Cellular Respiration. Introduction Cellular respiration is the procedure of changing the chemical energy of organic molecules into a type that can be used by organisms. Glucose may be oxidized completely if an adequate amount of oxygen is present. Equation For Cellular Respiration.
6. In cellular respiration, electrons are not transferred directly from glucose to oxygen. Each electron is coupled with a proton to form a hydrogen atom. Following the movement of hydrogens allows you to follow the flow of electrons. Lab 6: Cellular Respiration.
Read more. AP Biology Reading Guide Chapter 9 - Pearsoncmg. Read more.